SHIC funding porcine sapelovirus diagnostic tool development
Emerging virus was isolated in a diagnostic specimen from a U.S. swine farm.
National Hog Farmer
Nov 16, 2021
The Swine Health Information Center (SHIC) maintains a priority on diagnostics of swine diseases as part of its mission to protect and enhance the health of the U.S. swine herd. Consequently, SHIC funded a project for genetic characterization and diagnostic tool development for an emerging porcine sapelovirus (PSV). This emerging virus was isolated in a diagnostic specimen from a U.S. swine farm and designated as PSV KS18-01.
In work done at Kansas State University and the University of Illinois, a full-length genome sequence was obtained through next-generation sequencing. Phylogenetic analysis showed that the virus is more closely related to two Japanese strains but is distantly related to two known U.S. strains. PSV specific diagnostic tools were developed, including the monoclonal antibodies again VP1 and VP2, and a VP1-VP2 antigen-based indirect ELISA. Using this assay, the dynamic response of PSV antibody was investigated in a group of post-weaned pigs that were naturally exposed with PSV.
The availability of the PSV isolate (KS18-01) and the specific diagnostic reagents and assays provide important tools for PSV control and prevention, noted SHIC.
Porcine Sapelovirus (PSV), previously named as porcine enterovirus 8, belongs to the genus Sapelovirus in the family Picornaviridae. PSV is a non-enveloped, positive-sense single-stranded RNA virus...